Fig. 4
From: Early life diet conditions the molecular response to post-weaning protein restriction in the mouse
Post-weaning protein restriction leads to an altered small RNA profile in sperm but only when there has been no previous PR exposure. a The percentage of mappable reads falling into each class of small RNA, including transfer RNA (tRNA) fragments (tRF), small nuclear RNA (snRNA), small nucleolar RNA, ribosomal RNA (rRNA), Piwi-interacting RNA (piRNA), mitochondrial tRNA (Mt tRNA), mitochondrial ribosomal RNA (Mt rRNA), microRNA and long intergenic non-coding RNA (lincRNA). The percentage of unannotated reads are marked in grey and all other reads (processed transcripts and mRNA) are marked in beige. Fisher’s exact test for count data was used to assess the differences in distribution across the classes between the CTCT group and the CTPR group (adjusted P = 0.001), PRCT group (adjusted P = 0.09), and PRPR group (adjusted P = 0.99). P values were adjusted for n = 3 tests using Bonferroni’s method. b Differential expression analysis showed no genome-wide significant changes in abundances of tRNA-derived fragments between the CTCT group and any of the other groups (CTPR = blue, PRCT = orange, PRPR = red). The dotted line in each plot represents the nominal significance level (P = 0.05)