Fig. 4

LSTM optical sectioning. a x-z maximum intensity projections of an image stack acquired from human brain tissue, shown in (b), stained with DAPI. The camera rolling shutter exposure time determines the effective slit (rolling shutter) width (0.1–1 ms, i.e., 66–665 μm on the sCMOS sensor and 6–60 μm on the sample. The images were acquired using two different scanning modes: LSTM 1-axis scan (1-AS) and LSTM 2-axis scan (2-AS, default). Total frame exposure was 20 ms for all the images. As evident, the 2-AS mode allows for uniform planar illumination for achieving quantitative imaging, and the axial resolution decreases with increased rolling shutter exposure. All scale bars are 100 μm. b LSTM imaging of a large thick section of cleared human brain tissue (~ 10.5 mm × 14.1 mm × 3 mm) stained with DAPI. We used 0.5-ms rolling shutter exposure settings and 20 ms for entire frame exposure to acquire this dataset. Scale bar is 1 mm