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Table 2 Summary of imaging experiments reported in this study

From: Light sheet theta microscopy for rapid high-resolution imaging of large biological samples

Samples

Fig. No.

Label

Det. objective

Illum. objective

Imaging volume dimensions

No. of images/raw data

Imaging time

Thick human brain tissue

4

DAPI

10×/0.6NA/8mmWD

4×/0.28NA/28.5WD

~ 10.5 mm × 14.1 mm × 3 mm

116,736/~ 0.97 TB

~ 2.7 h

Mouse brain with attached spinal cord

5a

Thy1-eYFP

10×/0.6NA/8mmWD

4×/0.28NA/28.5WD

11.8 mm × 27.6 mm × 5.2 mm

388,687/~ 3.3 TB

~ 9 h

Thick mouse brain slice

5b

Thy1-eYFP

10×/0.6NA/8mmWD

4×/0.28NA/28.5WD

~ 9.6 mm × 13.5 mm × 5.34 mm

256,560/

~ 2.1 TB

~ 5.9 h

Thick mouse brain slice

5c

Thy1-eYFP

25×/1.0NA/8 mm/WD

4×/0.28NA/28.5WD

6 mm × 9.6 mm × 1.9 mma

211,616/

~ 1.8 TB

~ 4.9 h

Thick rat brain slice

6a, b

Tomato lectin

10×/0.6NA/8mmWD

4×/0.28NA/28.5WD

~ 20 mm × 16.5 mm × 3.6 mm deepb

285,821 slices/2.4 TB

~ 6.6 h

expanded (~ 4×) mouse brain slice

6c

Thy1-eYFP

10×/0.6NA/8mmWD

4×/0.28NA/28.5WD

33.2 mm × 19.3 mm × 2 mmc

723,200/

~ 6 TB

~ 22 h

Hydra live imaging

7

GCaMP6s

10×/0.6NA/8mmWD

4×/0.28NA/28.5WD

1.2 mm × 1.2 mm × 0.136 mm

23,001/~ 193 GB

~ 1 h live imaging

  1. Summary of the datasets reported in this report.
  2. aThe image volume acquired was ~ 6 mm × 9.6 mm × 1.9 mm; however, due to constraints of high-quality volume rendering, a smaller (0.5-mm-thick) subset was used for the rendering shown in Fig. 5c
  3. bThe approximate imaging volume was ~ 20 mm × 16.5 mm × 3.6 mm, and a few ~ 5-mm-deep image stacks were acquired to demonstrate the imaging depth in Fig. 6b
  4. cThe imaging volume acquired was ~ 33.2 mm × 19.3 mm × 2 mm to ensure complete coverage of ~ 1-mm-thick expanded non-rigid tissue
  5. TB terabytes, GB gigabytes, h hours