From: Light sheet theta microscopy for rapid high-resolution imaging of large biological samples
Samples | Fig. No. | Label | Det. objective | Illum. objective | Imaging volume dimensions | No. of images/raw data | Imaging time |
---|---|---|---|---|---|---|---|
Thick human brain tissue | 4 | DAPI | 10×/0.6NA/8mmWD | 4×/0.28NA/28.5WD | ~ 10.5 mm × 14.1 mm × 3 mm | 116,736/~ 0.97 TB | ~ 2.7 h |
Mouse brain with attached spinal cord | 5a | Thy1-eYFP | 10×/0.6NA/8mmWD | 4×/0.28NA/28.5WD | 11.8 mm × 27.6 mm × 5.2 mm | 388,687/~ 3.3 TB | ~ 9 h |
Thick mouse brain slice | 5b | Thy1-eYFP | 10×/0.6NA/8mmWD | 4×/0.28NA/28.5WD | ~ 9.6 mm × 13.5 mm × 5.34 mm | 256,560/ ~ 2.1 TB | ~ 5.9 h |
Thick mouse brain slice | 5c | Thy1-eYFP | 25×/1.0NA/8 mm/WD | 4×/0.28NA/28.5WD | 6 mm × 9.6 mm × 1.9 mma | 211,616/ ~ 1.8 TB | ~ 4.9 h |
Thick rat brain slice | 6a, b | Tomato lectin | 10×/0.6NA/8mmWD | 4×/0.28NA/28.5WD | ~ 20 mm × 16.5 mm × 3.6 mm deepb | 285,821 slices/2.4 TB | ~ 6.6 h |
expanded (~ 4×) mouse brain slice | 6c | Thy1-eYFP | 10×/0.6NA/8mmWD | 4×/0.28NA/28.5WD | 33.2 mm × 19.3 mm × 2 mmc | 723,200/ ~ 6 TB | ~ 22 h |
Hydra live imaging | 7 | GCaMP6s | 10×/0.6NA/8mmWD | 4×/0.28NA/28.5WD | 1.2 mm × 1.2 mm × 0.136 mm | 23,001/~ 193 GB | ~ 1 h live imaging |