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Table 2 Summary of imaging experiments reported in this study

From: Light sheet theta microscopy for rapid high-resolution imaging of large biological samples

Samples Fig. No. Label Det. objective Illum. objective Imaging volume dimensions No. of images/raw data Imaging time
Thick human brain tissue 4 DAPI 10×/0.6NA/8mmWD 4×/0.28NA/28.5WD ~ 10.5 mm × 14.1 mm × 3 mm 116,736/~ 0.97 TB ~ 2.7 h
Mouse brain with attached spinal cord 5a Thy1-eYFP 10×/0.6NA/8mmWD 4×/0.28NA/28.5WD 11.8 mm × 27.6 mm × 5.2 mm 388,687/~ 3.3 TB ~ 9 h
Thick mouse brain slice 5b Thy1-eYFP 10×/0.6NA/8mmWD 4×/0.28NA/28.5WD ~ 9.6 mm × 13.5 mm × 5.34 mm 256,560/
~ 2.1 TB
~ 5.9 h
Thick mouse brain slice 5c Thy1-eYFP 25×/1.0NA/8 mm/WD 4×/0.28NA/28.5WD 6 mm × 9.6 mm × 1.9 mma 211,616/
~ 1.8 TB
~ 4.9 h
Thick rat brain slice 6a, b Tomato lectin 10×/0.6NA/8mmWD 4×/0.28NA/28.5WD ~ 20 mm × 16.5 mm × 3.6 mm deepb 285,821 slices/2.4 TB ~ 6.6 h
expanded (~ 4×) mouse brain slice 6c Thy1-eYFP 10×/0.6NA/8mmWD 4×/0.28NA/28.5WD 33.2 mm × 19.3 mm × 2 mmc 723,200/
~ 6 TB
~ 22 h
Hydra live imaging 7 GCaMP6s 10×/0.6NA/8mmWD 4×/0.28NA/28.5WD 1.2 mm × 1.2 mm × 0.136 mm 23,001/~ 193 GB ~ 1 h live imaging
  1. Summary of the datasets reported in this report.
  2. aThe image volume acquired was ~ 6 mm × 9.6 mm × 1.9 mm; however, due to constraints of high-quality volume rendering, a smaller (0.5-mm-thick) subset was used for the rendering shown in Fig. 5c
  3. bThe approximate imaging volume was ~ 20 mm × 16.5 mm × 3.6 mm, and a few ~ 5-mm-deep image stacks were acquired to demonstrate the imaging depth in Fig. 6b
  4. cThe imaging volume acquired was ~ 33.2 mm × 19.3 mm × 2 mm to ensure complete coverage of ~ 1-mm-thick expanded non-rigid tissue
  5. TB terabytes, GB gigabytes, h hours