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Fig. 5 | BMC Biology

Fig. 5

From: Using in vivo oxidation status of one- and two-component redox relays to determine H2O2 levels linked to signaling and toxicity

Fig. 5

Kinetics of Tpx1, Trx1, Pap1, and HA-OxyR in wild-type cells upon sub-toxic and toxic concentrations of H2O2. a Cultures of strain AD29 (WT expressing HA-OxyR) were treated or not with 0.1 mM (left panels) or 0.5 mM (right panels) H2O2 for the indicated times, and protein extracts were obtained and processed as described in Fig. 1b, using antibodies against Tpx1 (ox. Tpx1 dimer and red. Tpx1 monomer), sulfinylated Tpx1 (Tpx1-SO2H), Trx1 (red. Trx1 and ox. Trx1), Pap1 (red. Pap1 and ox. Pap1), or HA (red. HA-OxyR and ox. HA-OxyR). b ,c Average of biological triplicates (b) or duplicates (c) of the experiment described in (a) are represented in curve graphs. Error bars (standard error of the mean) from the triplicates are shown. ox. oxidized, red. reduced, WT wild type

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