Using in vivo oxidation status of one- and two-component redox relays to determine H2O2 levels linked to signaling and toxicity

Table 1 Determination of pseudo-first-order rate constants for the extracellular removal of H₂O₂ and determination of catalase-driven gradients

Extracellular removal of H₂O₂ (k_cons) (s^− 1)
	H₂O₂ = 0.5 mM	H₂O₂ = 0.1 mM
Wild type	0.16	0.27
Δtpx1	0.18	0.22
Δctt1	0	0.24
Catalase-driven gradient
	k_cons (s⁻¹)	k_{intracellular} (s^− 1)	Gradient
Wild type	0.16	6.0	38
Δtpx1 (H₂O₂ = 0.5 mM)	0.18	6.0	33
Δtpx1 (H₂O₂ = 0.1 mM)	0.22	6.0	27

ISSN: 1741-7007