Skip to main content

Table 4 Analysis of reduction rates of heterologous expressed OxyR in Schizosaccharomyces pombe

From: Using in vivo oxidation status of one- and two-component redox relays to determine H2O2 levels linked to signaling and toxicity

 

[H2O2] (μM)

 

Oxidation at steady state (5 min)b

kswitch-off (s− 1)c

Strain

Extracellular

Intracellulara

Gradient

OxyR

Tpx1

OxyR

Tpx1

Δtrx1

2

0.048

42

28%

 

0.017

 

Δtrx1

5

0.12

42

89%

 

0.002

Δtrx3

20

0.12

160

24%

 

0.056

Δtrx3

50

0.31

160

77%

 

0.013

Wild type

20

0.075

267

17%

46%

0.050

0.85

Wild type

50

0.19

267

58%

71%

0.019

0.79

  1. aIntracellular concentration of H2O2 is calculated from the gradients between extracellular and intracellular H2O2 concentration determined from OxyR oxidation profiles
  2. bThe oxidation values for OxyR are extracted from experiments like those in Additional file 2: Figure S2 and the fitting shown in Additional file 3: Figure S3a, while Tpx1 oxidation values are an average of several biological replicates of experiments, like those of Additional file 2: Figure S2b
  3. cThe pseudo-first-order rate constant characterizing the reduction of thiol proteins is determined from the steady-state Eqs. 2 and 3, assuming that the second-order rate constants for the reaction of H2O2 with Tpx1 and OxyR are 1 × 107 M− 1 s− 1 and 1.4 × 105 M− 1 s− 1, respectively