Fig. 4
From: Enzyme intermediates captured “on the fly” by mix-and-inject serial crystallography
Concentration profile of the catalytic BlaC reaction with CEF as simulated with realistic parameters and a kinetic mechanism as discussed. The solid lines are calculated with τD = 15 ms, the dashed lines with τD = 1.5 ms. Black lines: free enzyme (E). Green lines: enzyme substrate complex (ES). Blue lines: enzyme acyl intermediate complex (E-CFO*). Red lines: product P (CFO, inactive CEF without leaving group, lactam ring open), released from the enzyme. Black dots: time delays are shown together with the approximate expected ratio of CEF to E-CFO*