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Table 1 Mixing parameters for each time point. The buffer contained 200–300 mmol/L CEF in either 1.0 mol/L sodium phosphate (shard crystal form), pH 5, or in 100 mmol/L sodium acetate, pH 5 (needle crystal form). The superscript and subscript numbers in the second column indicate that deviations to shorter times are different from those to longer times

From: Enzyme intermediates captured “on the fly” by mix-and-inject serial crystallography

Nominal time point (ms) Mixing time (ms) Delay time (ms) Sample flow (μL/min) Buffer flow (μL/min) Constriction diameter (μm)
30 \( {5}_{-3}^{+6} \) 42 ± 2 4.0 ± 0.5 66.0 ± 0.6 75 ± 1
100 \( {10}_{-8}^{+13} \) 114 ± 4 10.0 ± 0.5 70.0 ± 0.7 75 ± 1
500 \( {7}_{-5}^{+10} \) 510 ± 20 8.0 ± 0.5 32.0 ± 0.5 50 ± 1
2000 \( {15}_{-11}^{+20} \) 2300 ± 50 10.0 ± 0.5 45.0 ± 0.5 75 ± 1