Table 1 Mixing parameters for each time point. The buffer contained 200–300 mmol/L CEF in either 1.0 mol/L sodium phosphate (shard crystal form), pH 5, or in 100 mmol/L sodium acetate, pH 5 (needle crystal form). The superscript and subscript numbers in the second column indicate that deviations to shorter times are different from those to longer times
From: Enzyme intermediates captured “on the fly” by mix-and-inject serial crystallography
Nominal time point (ms) | Mixing time (ms) | Delay time (ms) | Sample flow (μL/min) | Buffer flow (μL/min) | Constriction diameter (μm) |
|---|---|---|---|---|---|
30 | \( {5}_{-3}^{+6} \) | 42 ± 2 | 4.0 ± 0.5 | 66.0 ± 0.6 | 75 ± 1 |
100 | \( {10}_{-8}^{+13} \) | 114 ± 4 | 10.0 ± 0.5 | 70.0 ± 0.7 | 75 ± 1 |
500 | \( {7}_{-5}^{+10} \) | 510 ± 20 | 8.0 ± 0.5 | 32.0 ± 0.5 | 50 ± 1 |
2000 | \( {15}_{-11}^{+20} \) | 2300 ± 50 | 10.0 ± 0.5 | 45.0 ± 0.5 | 75 ± 1 |