Fig. 5

REDD1 controls Hexokinase II (HKII) mitochondrial localization. a Akt T308 (WT n = 8, WT-DEX n = 9, KO n = 7, and KO-DEX n = 8), GSK3 S9 (WT and KO n = 8, and WT-DEX and KO-DEX n = 7) and HKII (WT, WT-DEX, and KO-DEX n = 7 and KO n = 8) protein expression in total lysate and b cytosolic (C) and crude mitochondrial (cM) subcellular localization of HKII in WT and REDD1 KO mice acutely treated with 1 mg/kg of dexamethasone (DEX; WT n = 7, WT-DEX n = 6, KO n = 3, and KO-DEX n = 4). c Representative pictures and corresponding quantifications of proximity ligation assay between VDAC and HKII with either control siRNA or siRNA against REDD1 in human primary myoblasts (n = 7 per condition from one culture). d Glycogen content in gastrocnemius of WT (n = 11) and REDD1 KO mice (n = 13). $p < 0.05 and $$$p < 0.001 vs. corresponding untreated group by two-way ANOVA and Fisher post-hoc test (a, b). *p < 0.05 and ***p < 0.001 vs. WT or control siRNA by unpaired t-test (c, d). C cytosol, cM crude mitochondria, CS citrate synthase, DEX dexamethasone, KO knockout, VDAC voltage-dependent anion channel, WT wild type