Fig. 2

YAC128 spine instability is predominantly MSN intrinsic. WT, YAC128, and chimeric co-cultures generated at a 1:3 CS ratio were processed at DIV21 for DARPP32 immunocytochemistry, imaging, and spine analysis. (a) Sample images of DARPP32-stained spines in pure or chimeric co-cultured MSNs (scale bar = 5 μm). (Bi) Total spine density values in chimeric cultures are similar to pure cultures of the same MSN genotype. (Bii) Mature mushroom and (Biii) immature spine numbers are affected by both striatal (STR) and cortical (CTX) mHTT expression [n = 32(4); one-way ANOVA with Bonferroni post-hoc analysis; **p < 0.01, ***p < 0.001]. Student’s t test was used to compare WT STR/WT CTX and WT STR/Y128 CTX [n = 32(4); Student’s t test; #p < 0.05]