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Fig. 5 | BMC Biology

Fig. 5

From: Harnessing single-cell genomics to improve the physiological fidelity of organoid-derived cell types

Fig. 5

Chemically induced Paneth cells (CI-PCs) are functional in response to host and microbial stimuli. a Supernatant LYZ from 24-h basal and 10 μm CCh-stimulated LYZ cells at varying number of days in ENR + CD culture (top). DNA content from matched samples (bottom) (n = 8 well replicates; SEM error bars too small to visualize). b Supernatant LYZ from 6-day ENR + CD collected basally and following 10 μm CCh stimulation for 0.5, 2, 4, 6, and 24 h (top). DNA content from matched samples basally and following 10 μm CCh stimulation (bottom) (n = 8 well replicates). c 24-h basal (non-stimulated) and 10 μm CCh-stimulated LYZ secretion in 6-day ENR + CD versus ENR and ENR + CV (n = 8 well replicates; two-way ANOVA with multiple comparison test; ns non-significant, * adj. p < 0.05, **** adj. p < 0.0001). d 4-h co-culture of freshly passaged 6-day ENR and ENR + CD cells and select gram-negative and gram-positive aerobic bacteria (n = 13 well replicates; two-way ANOVA with multiple comparison test, * adj. p < 0.05, *** adj. p < 0.001, **** adj. p < 0.0001). e Normalized cellular viability, caspase activity per viable cell, and cytotoxicity per viable cell from 24-h and 48-h ENR and ENR + CD co-cultures at specified mixing ratios (n = 9 well replicates from three biological donors; one sample t test,* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001)

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