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Fig. 7 | BMC Biology

Fig. 7

From: Ultrastructural analysis of mitotic Drosophila S2 cells identifies distinctive microtubule and intracellular membrane behaviors

Fig. 7

MT organization in prometaphase and metaphase S2 cells. a Examples of transverse sections of MTs in prometaphase and metaphase cells. In the bottom panels, MTs are pseudo-colored in red. In the PM1 cell shown, MTs are not bundled. In the PM2, PM3, PM4, and metaphase (M) cells, MTs are organized in bundles, and the average distance between bundled MTs decreases during the progression from early prometaphase to metaphase. Scale bar: 0.1 μm. b Frequencies of unbundled MTs (1–3 MTs) and of MTs included in bundles of different sizes (4–9, 10–15, 16–21, 22–27, 28–33, and 34–45 MTs) observed in transverse sections through the chromosomes of prometaphase and metaphase cells. All MT bundles were taken into account, including those outside the metaphase plate. c Quantification of the distances between MTs within bundles (10 bundles were analyzed for each stage). The average distances observed in PM3, PM4, and metaphase (M) cells are significantly smaller than those seen in PM1 and PM2 cells (* and **, p < 0.05 and 0.01, respectively; ANOVA with post-hoc Tukey HSD). d Frequencies of MT bundle sizes (number of MTs per bundle) from early prometaphase to metaphase (74, 102, and 101 bundles were analyzed for the PM1 + PM2, PM3 + PM4, and M stages, respectively). M metaphase

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