Fig. 3

Conditional KO ssODN (a) and lssDNA (b) targeting attempts. Each donut chart represents the summation of each allele type for all F0 mice genotyped, by ODN donor. In the center of the chart is the total number of F0 mice genotyped. Percentages of each allele type of the total number of mice genotyped are listed on each segment. 5′ and 3′ loxP: Includes animals genotyped for both 5′ and 3′ loxP sites, irrespective of the presence of any additional alleles (e.g., animals with 5′ loxP, 3′ loxP and a null allele detected); Null allele: Includes animals genotyped for a null allele, which may also have a single HDR and/or NHEJ indel event; Single HDR event: Includes animals genotyped for a single HDR event with or without additional indel events; NHEJ Indel event: Animals in which only indel alleles were observed. a Summary of genotypes identified from ssODN targeting attempts, and symmetric and asymmetric homology arm designs. b Summary of genotypes identified from four lssDNA targeting attempts