Fig. 6

a Copy number data from quantitative SYBR Green PCR assays for Abat, Kctd7, Slc2a12, and Uqcr10, using primers following the design introduced in Fig. 2d for both 5′ and 3′ loxP ssODN donors. All mice screened for each conditional null attempt were heterozygous conditional null mice from the N1 generation that were sequence confirmed for the conditional allele. Negative control samples were CRISPR-targeted N1 mice that were wild-type for the allele being analyzed. b, c Copy number data from TaqMan^® Copy Number assays for (b) Slc2a12 and Smc1a (paired ssODN conditional null targeting attempts) and (c) Eif2s2 and Cd44 (single lssDNA conditional null targeting attempts) using N1 progeny from a single founder. Genotypes are listed with the mouse ID. Animals with an asterisk to the right of the genotype were sequence-confirmed for the conditional null allele. Of note, Smc1a is located on the X chromosome, thereby males only have one copy of the gene. As in (a), negative control samples were CRISPR-targeted N1 mice that were wild-type for the allele being analyzed