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Table 1 Transmittance of 2 loxP sites to N1 generation

From: Comparative analysis of single-stranded DNA donors to generate conditional null mouse alleles

Donor

2 loxP Loci Bred

loxP in cis

In cis, NOT mutated

Overall success

2× ssODNs

22

18 (81%)a

14 (77%)b

44%c

 Symmetric

15

14 (93%)

10 (71%)

45%

 Asymmetric

7

4 (57%)

4 (100%)

40%

  1. aPercentage represents number of conditional alleles with loxP in cis over total 2 loxP loci bred; bPercentage represents number of loci with no mutations detected over all loci with loxP in cis. cPercentage represents number of loci with no mutations detected over all loci attempted
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BMC Biology

ISSN: 1741-7007

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