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Fig. 2 | BMC Biology

Fig. 2

From: Application of long single-stranded DNA donors in genome editing: generation and validation of mouse mutants

Fig. 2

GckrP446L point mutation. Different designs of reagents for genome editing employing (a) oligonucleotides or (b) a lssDNA donor. Donors were designed containing both coding (in red) and silent mutations (in black) that prevent reprocessing of engineered alleles in accordance with the selected sgRNAs. Guide sequences are named sgRNAs. The shared colour coding of guides and donors highlights reagents injected within the same mix

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