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Table 3 Generation of a GckrP446L point mutation

From: Application of long single-stranded DNA donors in genome editing: generation and validation of mouse mutants

       F0 with:
MS Donor type Guide ID(s) Donor ID Embryos transferred F0 biopsied (birth rate) Mutation Correct mutation SM only SM only and rearranged NHEJ alleles Random integration
1 ssODN 20 Gckrdonor_20 80 12 (15%) 0 0 0 0 0 n.d.
2 ssODN 3 Gckrdonor_2 80 21 (26%) 12 0 2 2 9 n.d.
3 ssODN 3 Gckrdonor_2 70 13 (19%) 4 0 0 0 4 n.d.
4 ssODN 3 Gckrdonor_2 135 18 (13%) 9 0 2 1 7 n.d.
5 ssODN 3 Gckrdonor_2 42 10 (23%) 3 0 0 0 3 n.d.
6 ssODN 3 Gckrdonor_3 121 8 (7%) 5 0 1 1 3 n.d.
7 ssODN 3 Gckrdonor_3 112 8 (7%) 3 0 0 0 3 n.d.
1 lssDNA 5.2, 3.1 Gckr_P446L_lss 210 22 (10%) 14 8 1 2 7 0/2
  1. The table shows the numbers of embryos and animals involved in mutagenesis attempts employing the injection of CRISPR/Cas9 reagents and oligonucleotides or lssDNA donors. The percentage of transferred embryos yielding live animals at weaning is shown in parentheses. The outcome of these attempts is also summarized. Note that sgRNA_20 was employed for the first microinjection session with ssODN_20 and substituted to sgRNA_3 and relevant donor ssODNs for subsequent sessions, as it was confirmed to be inactive. Sequencing data from this project are displayed in Fig. 4 (additional raw sequencing data are provided in Additional file 16)
  2. MS microinjection session, n.d. not determined SM silent mutation