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Fig. 6 | BMC Biology

Fig. 6

From: Dehydration triggers ecdysone-mediated recognition-protein priming and elevated anti-bacterial immune responses in Drosophila Malpighian tubule renal cells

Fig. 6

Functional immunity enhanced by desiccation stress response requires EcR and PGRP-LC in Malpighian tubules (MTs). a–e KaplanMeier survival plots of adult females sterile pricked or infected with Ecc15, with or without prior desiccation (des) and either no recovery, 3 h recovery, or 6 h recovery. Genotypes: a yw; b +/EcR(RNAi); c +/PGRP-LC(RNAi); d c324-gal4/UAS-EcR(RNAi); e c324-gal4/UAS-PGRP-LC(RNAi). Each survival curve is from a combined lifetable of two cohorts tested at different times, each cohort initiated with 90 females. For survival analyses (right censored log-rank test) conducted with six pair-wise comparisons within each genotype; curves with different letters differ significantly by at least p < 0.02 (details in Additional file 9: Table S1). f Microbial load (colony-forming units) 48 h after Ecc15 infection of adult females across treatments by desiccation (0 or 2 h) and post-desiccation recovery (0, 3, 6 h). Genotypes as in (a–e): wildtype controls (yw, +/EcR, +/PGRP-LC) and EcR or PGRP-LC knock-down in principal cells (c324 > RNAi). For each genotype, three independent samples were prepared from each treatment group, in both cohort trials (displaying mean and SD of all six observations). CFU compared by ANOVA with Sidak post-hoc comparison, bars indicate differences with p < 0.05, see details in Additional file 10: Table S2

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