Table 2 Plasmids used in this study
From: Determinants of the cytosolic turnover of mitochondrial intermembrane space proteins
Name (lab ID no.) | Backbone and description | Reference |
|---|---|---|
pRS414 | pRS414 | [101] |
pYEp96–6HIS-Ub (161) | pYEp96-CUP1PR-6His-tagged ubiquitin | [103] |
pRS425 | pRS425 | [101] |
pLK1 (589) | pRS425-CUP1PR-6His-Ub | This study |
pESC-URA | pESC-URA | Agilent |
pAG3 (55) | pESC-URA-GAL10PR-Cox12FLAG | [31] |
pPB36.1 (471) | pESC-URA-CUP1PR-Cox12FLAG | This study |
pEG1 (103) | pESC-URA-GAL10PR-Cox12C27S-FLAG | This study |
pEG2 (104) | pESC-URA-GAL10PR-Cox12C37S-FLAG | This study |
pEG3 (105) | pESC-URA-GAL10PR-Cox12C48S-FLAG | This study |
pEG4 (106) | pESC-URA-GAL10PR-Cox12C59S-FLAG | This study |
pLK2 (590) | pESC-URA-GAL10PR-Cox12C-FREE-FLAG | This study |
pPB37.1 (472) | pESC-URA-CUP1PR-Cox12C-FREE-FLAG | This study |
pPB32 (467) | pESC-URA-GAL10PR-Cox12K-FREE-FLAG | This study |
pPB40.1 (613) | pESC-URA-CUP1PR-Cox12K-FREE-FLAG | This study |
pLK3 (591) | pESC-URA-GAL10PR-Cox12K25-FLAG | This study |
pLK4 (592) | pESC-URA-GAL10PR-Cox12K36-FLAG | This study |
pLK5 (593) | pESC-URA-GAL10PR-Cox12K41-FLAG | This study |
pPB33 (468) | pESC-URA-GAL10PR-Cox12K73-FLAG | This study |
pRS415 | pRS415 | [101] |
pPB19.1 (200) | p415-GDPPR-mCherry-sfGFP | This study |
pPB20.1 (201) | p415-GDPPR-Cox12-mCherry-sfGFP | This study |
pPB24.1 (385) | p415-GDPPR-Cox12C-FREE-mCherry-sfGFP | This study |
pPB30.1 (391) | p415-GDPPR-Cox17-mCherry-sfGFP | This study |
pPB21.1 (202) | p415-GDPPR-Pet191-mCherry-sfGFP | This study |
pPB25.1 (386) | p415-GDPPR-Tim9-mCherry-sfGFP | This study |
pPB28.1 (389) | p415-GDPPR-Tim10-mCherry-sfGFP | This study |
pMaM98 (158) | p415-GDPPR-Ubi-I-mCherry-sfGFP | [51] |
pMaM99 (159) | p415-GDPPR-Ubi-M-mCherry-sfGFP | [51] |
pMaM100 (160) | p415-GDPPR-Ubi-F-mCherry-sfGFP | [51] |