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Table 2 Plasmids used in this study

From: Determinants of the cytosolic turnover of mitochondrial intermembrane space proteins

Name (lab ID no.) Backbone and description Reference
pRS414 pRS414 [101]
pYEp96–6HIS-Ub (161) pYEp96-CUP1PR-6His-tagged ubiquitin [103]
pRS425 pRS425 [101]
pLK1 (589) pRS425-CUP1PR-6His-Ub This study
pESC-URA pESC-URA Agilent
pAG3 (55) pESC-URA-GAL10PR-Cox12FLAG [31]
pPB36.1 (471) pESC-URA-CUP1PR-Cox12FLAG This study
pEG1 (103) pESC-URA-GAL10PR-Cox12C27S-FLAG This study
pEG2 (104) pESC-URA-GAL10PR-Cox12C37S-FLAG This study
pEG3 (105) pESC-URA-GAL10PR-Cox12C48S-FLAG This study
pEG4 (106) pESC-URA-GAL10PR-Cox12C59S-FLAG This study
pLK2 (590) pESC-URA-GAL10PR-Cox12C-FREE-FLAG This study
pPB37.1 (472) pESC-URA-CUP1PR-Cox12C-FREE-FLAG This study
pPB32 (467) pESC-URA-GAL10PR-Cox12K-FREE-FLAG This study
pPB40.1 (613) pESC-URA-CUP1PR-Cox12K-FREE-FLAG This study
pLK3 (591) pESC-URA-GAL10PR-Cox12K25-FLAG This study
pLK4 (592) pESC-URA-GAL10PR-Cox12K36-FLAG This study
pLK5 (593) pESC-URA-GAL10PR-Cox12K41-FLAG This study
pPB33 (468) pESC-URA-GAL10PR-Cox12K73-FLAG This study
pRS415 pRS415 [101]
pPB19.1 (200) p415-GDPPR-mCherry-sfGFP This study
pPB20.1 (201) p415-GDPPR-Cox12-mCherry-sfGFP This study
pPB24.1 (385) p415-GDPPR-Cox12C-FREE-mCherry-sfGFP This study
pPB30.1 (391) p415-GDPPR-Cox17-mCherry-sfGFP This study
pPB21.1 (202) p415-GDPPR-Pet191-mCherry-sfGFP This study
pPB25.1 (386) p415-GDPPR-Tim9-mCherry-sfGFP This study
pPB28.1 (389) p415-GDPPR-Tim10-mCherry-sfGFP This study
pMaM98 (158) p415-GDPPR-Ubi-I-mCherry-sfGFP [51]
pMaM99 (159) p415-GDPPR-Ubi-M-mCherry-sfGFP [51]
pMaM100 (160) p415-GDPPR-Ubi-F-mCherry-sfGFP [51]