Fig. 1
From: Proteasomal degradation competes with Mia40-mediated import into mitochondria
Tandem fluorescent protein timer (tfT) approach to measure cytosolic protein turnover by the ubiquitin-proteasome system. a IMS proteins are tagged with a slow maturating mCherry and a fast maturating superfolder green fluorescent protein (sfGFP). The ratio of red (mCherry) to green (sfGFP) intensities correlates with protein stability. b Protein import of Mia40 substrates into the IMS competes with their ubiquitin-dependent degradation in the cytosol