Fig. 4

NMR analysis of SGTA constructs. a Cartoon representation of the different SGTA variants used for NMR comparison. b Overlaid ¹H-¹⁵N HSQC spectra of FL SGTA (black), NT (blue), TPR (red), and CT (green) constructs. c Chemical shift difference analysis of amide signals from N-terminal, TPR, and C-terminal region in the single domain context versus FL SGTA. d ¹⁵N - {¹H} heteronuclear NOE measurements for FL SGTA