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Table 1 Maximum targeting possible with 1, 2, or 3 gRNAs

From: Viral diversity is an obligate consideration in CRISPR/Cas9 designs for targeting the HIV reservoir

 

Subtype A

Subtype B

Subtype C

Group M

n

Single

Pair

Triplet

n

Single

Pair

Triplet

n

Single

Pair

Triplet

n

Single

Pair

Triplet

LTR

75

90.7

100.0

100.0

284

74.3

92.6

98.6

373

84.5

96.0

98.9

1216

70.6

83.0

88.8

gag

404

86.4

96.3

99.5

3280

80.9

95.2

98.5

1865

75.7

94.0

98.4

8453

71.1

88.2

95.5

pol

150

96.0

100.0

100.0

1750

88.4

98.6

99.8

878

84.6

97.6

99.9

4416

86.5

96.5

99.2

  1. n = number of sequences in the alignment; the remaining columns show the percentage (out of total sequences) that can be targeted with single, paired, or triplet gRNA combinations