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Table 1 Maximum targeting possible with 1, 2, or 3 gRNAs

From: Viral diversity is an obligate consideration in CRISPR/Cas9 designs for targeting the HIV reservoir

  Subtype A Subtype B Subtype C Group M
n Single Pair Triplet n Single Pair Triplet n Single Pair Triplet n Single Pair Triplet
LTR 75 90.7 100.0 100.0 284 74.3 92.6 98.6 373 84.5 96.0 98.9 1216 70.6 83.0 88.8
gag 404 86.4 96.3 99.5 3280 80.9 95.2 98.5 1865 75.7 94.0 98.4 8453 71.1 88.2 95.5
pol 150 96.0 100.0 100.0 1750 88.4 98.6 99.8 878 84.6 97.6 99.9 4416 86.5 96.5 99.2
  1. n = number of sequences in the alignment; the remaining columns show the percentage (out of total sequences) that can be targeted with single, paired, or triplet gRNA combinations