Fig. 1
From: FlashFry: a fast and flexible tool for large-scale CRISPR target design
Discovery and scoring of CRISPR target sites. FlashFry schematic. The genome of interest is scanned for targets that match the PAM of the specified CRISPR enzyme. These genomic targets are then aggregated and bit-encoded into a database of compressed bins, sorted by their prefix. This database can then be searched by comparing the prefix of a candidate target against the prefix of the bins, and bins within the allowed mismatch (orange) can be examined for individual off-targets in the genome. The resulting off-target list is aggregated and used by various scoring metrics