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Table 1 A sample of computational times (h:m:s) required to build a FlashFry database for versions of the Caenorhabditis elegans, human, mouse, and Drosophila melanogaster genomes for common CRISPR enzymes. All timing analyses were run with default FlashFry parameters on an Amazon r4.large instance, limited to 8 GB of memory limited and one CPU core

From: FlashFry: a fast and flexible tool for large-scale CRISPR target design

Genome CRISPR/Cas9 (NGG) CRISPR/Cas9 (NGG/NAG) Cpf1 (TTTN)
Caenorhabditis elegans—235 0:1:28 0:2:55 0:2:13
Human—hg38 1:15:15 2:42:27 0:56:23
Mouse—mm10 1:02:13 2:21:11 0:43:13
Drosophila melanogaster—BDGP6 0:2:38 0:5:01 0:2:14