Fig. 5
Opponent and non-opponent melanopsin-responsive units are readily identifiable under the stimulus paradigm used for pupillography. a, b Mean ± SEM baseline subtracted responses of S-ON/L-OFF (a; n = 7) and non-opponent (b; n = 9) MR units to 75% contrast cone isolating and all opsin stimuli delivered as for pupillography (Fig. 4). The sample tested here (n = 5 Opn1mwR mice) did not include any L-ON/S-OFF MR units. c, d Left: Mean ± SEM change in firing between ‘dim’ and ‘bright’ stimulus phases for all stimuli (averaged across full 5 s phase and both stimulus polarities) for S-ON/L-OFF (c) and non-opponent units (d) as above. Data analysed by Freidman’s ANOVA with Dunn’s multiple comparison test. Right: Mean ± SEM change in firing between ‘dim’ and ‘bright’ stimulus phases for 75% contrast stimuli targeting L + S cone opsins or all photoreceptors as a function of time since contrast step (averaged across both stimulus polarities as above). Data analysed by two-way RM ANOVA with Sidak’s post-tests. * and ** = P < 0.05 and 0.01 respectively