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Fig. 4 | BMC Biology

Fig. 4

From: Structural and functional characterization of protein–lipid interactions of the Salmonella typhimurium melibiose transporter MelB

Fig. 4

CD spectra and Tm determination. MelBSt was purified from varied lipid strains as described in Methods. a CD spectra. MelBSt at 10 μM of protein in a buffer containing 20 mM NaPi, 100 mM NaCl, 10% glycerol and 0.035% UDM was placed in 1 mm quartz cuvette in a temperature-controlled cell holder. The spectra were recorded between 200 and 260 nm and subtracted with corresponding buffer backgrounds. b The thermal denaturation profiles. The CD ellipticity changes was recorded at 210 nm each degree with temperature ramping 1 °C per minute

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