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Table 3 Binding affinities and kinetic rate constants for binding of Ang2-BD BC5 to immobilized integrins

From: Targeting the Tie2–αvβ3 integrin axis with bi-specific reagents for the inhibition of angiogenesis

Integrin

KD ± sem, nM

Kon1 ± sem (M−1 s−1) × 104

Koff1 ± sem (s−1) × 10−3

Kon2 ± sem (s−1) × 10−3

Koff2 ± sem (s−1) × 10−3

αvβ1

550 ± 90.1

1.14 ± 0.01

6.29 ± 0.17

3.04 ± 0.12

1.97 ± 0.03

αvβ5

65 ± 21.8

3.98 ± 0.03

2.59 ± 0.12

5.44 ± 0.21

0.35 ± 0.01

αvβ6

364 ± 70.8

2.29 ± 0.05

8.35 ± 0.45

3.2 ± 0.17

1.06 ± 0.03

αvβ8

138 ± 50.3

3.66 ± 0.03

5.05 ± 0.13

2.4 ± 0.12

2.15 ± 0.05

  1. Values in the table are means ± SEM. SPR sensorgram curves were fit to a two-state binding model