Fig. 2

Cluster analysis of samples based on the mean gene expression of each time point, for 596 differentially expressed genes (adjusted p value ≤ 0.05). a PCA plot of brood-swap and control samples. Percentages on each axis indicate the proportion of variance explained by the indicated principal component. The blue, brown, and green ellipses show the k-means cluster assignment. The color of each sample indicates the average ovary activation score as per [77]; 0 indicates no signs of ovary activation while 4 indicates fully developed eggs are present. Sample names are as per Fig. 1. b Heatmap showing Euclidean distances between all time points. The dendrogram was constructed using the average distances between time points. The blue and green color bar above the heatmap indicates average ovary activation score, as in a. Sample names are as per Fig. 1