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Fig. 2 | BMC Biology

Fig. 2

From: Synaptic coupling of inner ear sensory cells is controlled by brevican-based extracellular matrix baskets resembling perineuronal nets

Fig. 2

Localization of brevican and HAPLN1 in whole-mount preparations of the mouse cochlea. a, b Immunolabeling of brevican (BCAN, a) and HAPLN1 (b) revealed an extensive accumulation of these ECM molecules at IHCs. cf Double-immunolabeling with the Ribeye marker CtBP2 (red, c), the neurofilament marker SMI32 (red, d), and antibodies against GluR2/3 (red, e), and GluR4 (red, f) showed that brevican (green, c, d) and HAPLN1 (green, e, f) were expressed at the base of the IHCs in the region where the pre- and postsynaptic elements of IHC synapses are located. g, h Magnifications of indicated IHCs in d and f revealed a basket-like formation of ECM at IHCs, with brevican (green, g) tightly enclosing terminating fibers (SMI32, red, g) and HAPLN1 (green, h) forming gaps in which glutamate receptors are located (GluR4, red, h). a-h Maximum intensity projections of confocal stacks of whole-mount preparations from apical turns of organs of Corti. Nuclei are stained with DAPI (blue). In c, IHCs are marked with an anti-vGlut3 antibody (blue). In df, an exemplary IHC is indicated by the dashed outline. a, b Scale 100 μm. cf Scale 5 μm. g, h Scale 2 μm

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