Fig. 2

Parkin mutation/loss-of-function decreases cell-surface GluN1 levels. a Representative images of surface GluN1 staining (red) in 14–16 DIV hippocampal neurons expressing GFP, shParkin, shParkin-WT, shParkin-T240M, shParkin-R275W, shParkin-R334C, or shParkin-G430D constructs. Scale bar, 10 μm. b Quantification of cell-surface GluN1 intensity expressed as a fraction of shParkin-WT (n ≥ 50 fields of view per condition with > 100 GluN1 puncta per field, results confirmed in 4 independent experiments. ***P < 0.001, one-way ANOVA, error bars represent SEM). Scale bar, 10 μm. c Representative images of surface GluN1 staining (red) in 14–16 DIV Parkin KO hippocampal neurons expressing shParkin, shParkin-WT, shParkin-T240M, shParkin-R275W, shParkin-R334C or shParkin-G430D constructs, and non-transduced KO control. Scale bar, 10 μm. d Quantification of cell-surface GluN1 intensity expressed as a fraction of Parkin KO (n ≥ 50 fields of view per condition with > 100 GluA1 puncta per field, results confirmed in 2 independent experiments. ***P < 0.001, one-way ANOVA, error bars represent SEM)