Fig. 4

Parkin E3 ligase activity is required to maintain cell-surface NMDARs but not AMPARs. a Representative images of surface GluA1 staining (red) in 14–16 DIV hippocampal neurons expressing shParkin +/− WT, C431S, or W403A Parkin constructs. Scale bar, 10 μm. b Same condition as (a), but for surface GluN1 staining (red). Scale bar, 10 μm. c Quantification of cell- surface GluA1 intensity expressed as a fraction of shParkin control (n ≥ 40 fields of view per condition with > 100 GluA1 puncta per field, results confirmed in 3 independent experiments. ***P < 0.001, one-way ANOVA, error bars represent SEM). d Quantification of cell-surface GluN1 intensity expressed as a fraction of shParkin control (n ≥ 40 fields of view per condition with > 100 GluN1 puncta per field, results confirmed in 3 independent experiments. ***P < 0.001, one-way ANOVA, error bars represent SEM)