Fig. 9
From: Parkinson’s disease-linked Parkin mutations impair glutamatergic signaling in hippocampal neurons
Parkin mutation/knockout impairs the induction of LTD but not LTP. a Representative images of surface GluA1 staining (red) in 14–16 DIV Parkin KO hippocampal neurons expressing shParkin or shParkin-WT/-T240M/-R275W/-R334C/-G430D constructs, and non-transduced KO control under the control condition (no treatment) or after chemical LTP (cLTP) induction. Scale bar, 10 μm. b Quantification of the ratio of GluA1 intensity after cLTP induction to the control condition for neurons expressing the above Parkin constructs. c Same as (a), but for control condition or chemical LTD (cLTD) induction. Scale bar, 10 μm. d Quantification of the ratio of GluA1 intensity after cLTD induction to the control condition for neurons expressing above Parkin constructs. For panels (b) and (d), n ≥ 40 fields of view per condition with > 100 GluA1 puncta per field, results confirmed in 2 independent experiments. ***P < 0.001, one-way ANOVA, error bars represent SEM