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Fig. 1 | BMC Biology

Fig. 1

From: Histone variants H2A.Z and H3.3 coordinately regulate PRC2-dependent H3K27me3 deposition and gene expression regulation in mES cells

Fig. 1

H2A.Z is required for the proper genome-wide distribution of H3K27m3 in mES Cells. a Heat map for distributions of H3K27me3, H2A.Z, and H3.3 at the H3K27me3-enriched regions in R1 mES cells. The y-axis coordinates with 4,445 H3K27me3 peaks in mES cells. The zero point of x-axis corresponds with the center of each H3K27me3 peak and expands from 5 kb upstream to 5 kb downstream to form H3K27me3 peak regions. b Scatterplot for the average reads density of H3K27me3 (red line), H2A.Z (blue line), and H3.3 (green line) at the H3K27me3-enriched regions. c The schematic view of mononucleosomes immuno-precipitation (Mono-IP), stable overexpressed histone H2A, or H2A.Z mouse R1 ES cell line was generated and applied here. d Histone modifications associated with H2A- and H2A.Z-containing mononucleosomes (marked in c). The histone modifications preferentially associated with H2A.Z-containing mononucleosomes are highlighted in red. e Western blot to analyze the dynamic changes of the global level of H3K27me3 upon knockdown of H2A.Z in mES cells. f Heat map for dynamic changes of H3K27me3 and H2A.Z levels at the H3K27me3-enriched regions as shown in a upon knockdown of H2A.Z in mES cells. g Scatterplot for average reads densities of H2A.Z at the H3K27me3-enriched regions (as shown in a) in wild-type mES cells (referred as siNC) and H2A.Z knockdown mES cells (referred as siH2A.Z). h Scatterplot for average reads densities of H3K27me3 at the H3K27me3-enriched regions (as shown a) in wild-type mES cells (referred as siNC) and H2A.Z knockdown mES cells (referred as siH2A.Z)

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