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Fig. 3 | BMC Biology

Fig. 3

From: Histone variants H2A.Z and H3.3 coordinately regulate PRC2-dependent H3K27me3 deposition and gene expression regulation in mES cells

Fig. 3

Dynamic changes of chromatin compaction and H3K27me3 deposition upon knockdown of H2A.Z in mES cells. a Heat map for dynamic changes of MNase hypersensitivities (MHS) and H2A.Z levels around the H2A.Z peak regions after H2A.Z knocking down in mES cells. The y-axis coordinates with 44,112 high confident H2A.Z peaks in mES cells. The zero point of x-axis corresponds with the center of each H2A.Z peak and expands from 5 kb upstream to 5 kb downstream to form H2A.Z peak regions. Chromatin accessibility was measured by MNase hypersensitive sites which could be digested by micrococcal nuclease (MNase). b Scatterplot for average reads densities of H2A.Z around the H2AZ peak regions (as shown in a) in wild-type mES cells (referred as siNC) and H2A.Z knockdown mES cells (referred as siH2A.Z). c Scatterplot for average reads densities of MNase hypersensitivities (MHS) around H2AZ peak regions (as shown in a) in wild-type mES cells (referred as siNC) and H2A.Z knockdown mES cells (referred as siH2A.Z). d Heat map for the dynamic changes of H2A.Z, MNase hypersensitivities (MHS), and H3K27me3 levels around H2A.Z/H3K27me3-colocalized regions after H2A.Z knocking down in mES cells. The y-axis coordinates with 1,400 H2A.Z/H3K27me3-colocalized peaks in mES cells. The zero point of x-axis corresponds with the center of each peak and expands from 5 kb upstream to 5 kb downstream to form H2A.Z peak regions. eg Scatterplots for average reads densities of H2A.Z (panel e), MNase hypersensitivities (panel f), and H3K27me3 (panel g) around H3K27me3 peak regions (as shown in d) in wild-type mES cells (referred as siNC) and H2A.Z knockdown mES cells (referred as siH2A.Z)

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