Fig. 2

Reduced EED leads to epigenetic dysregulation of transposable elements in the paternal germline. a Major sequence classes identified with H3K27me3 enrichment identified by ChIP-seq in FACS purified E15.5 Eed^wt/wt (n = 4) and Eed^hypo/hypo (n = 4) germ cells. b Graphical summary of regions with loss or gain of H3K27me3 in Eed^hypo/hypo germ cells (n = 4) compared to Eed^wt/wt germ cells (n = 4). c Hypergeometric analysis of the expected and observed representation of sequences on which H3K27me3 was reduced (i) or increased (ii) in Eed^hypo/hypo (n = 4) compared to Eed^wt/wt (n = 4) male E15.5 germ cells, grouped by annotation. P values for over- and under-representation of each sequence category are shown, with P < 0.05 considered significant. d Chi-square analysis of the expected and observed representation of sequences on which H3K27me3 was increased (HOM > WT) or reduced (WT > HOM) in Eed^hypo/hypo (n = 4) compared to Eed^wt/wt (n = 4) male E15.5 germ cells, grouped by annotation. Intergenic sequences were significantly over-represented in sequences gaining H3K27me3, while intronic sequences were significantly under-represented in sequences losing H3K27me3 in Eed^hypo/hypo germ cells. e Fold enrichment of transcripts from LINE, intergenic, low complexity, SINE and protein-coding genes in germ cells from E15.5 Eed^hypo/hypo (n = 4) and Eed^wt/wt (n = 4) foetuses. *P < 0.05 (Fisher exact test). Dotted line illustrates no enrichment relative to random distribution