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Fig. 3 | BMC Biology

Fig. 3

From: The piRNA pathway responds to environmental signals to establish intergenerational adaptation to stress

Fig. 3

Intergenerational fitness effects of growth at altered temperatures. a Experimental design used for the competition assay. Two strains with either different fluorescence (unmarked or GFP-marked) or different genotypes (N2 or N2 containing introgressed SNPs from JU1580 on Chromosome IV) were grown at 20 °C or 25 °C and then synchronised using sodium hypochlorite treatment (bleaching). Equal number of F1s from two different parental treatments was then put in the same plate. All different combinations have been tested in parallel either in triplicate or in quintuplicate. Before starvation, animals were harvested and the proportions of each of the different strains measured using a fluorescent microscope (GFP-marked animals) or using pyrosequencing (SNP-marked animals). 200 to 400 animals were transferred to a fresh NGM plate and the proportions were measured similarly. b Intergenerational competition at 20 °C between animals derived from (left) unmarked P0 animals grown at 20 °C and GFP-marked P0 animals grown at 25 °C; (right) GFP-marked P0 animals grown at 20 °C and unmarked P0 animals grown at 25 °C. c Competition at 20 °C between animals derived from (left) unmarked P0 animals grown at 20 °C and SNP-marked P0 animals grown at 25 °C; (right) SNP-marked P0 animals grown at 20 °C and unmarked P0 animals grown at 25 °C. Y-axes show the proportion of unmarked animals after 1 transfer for all replicates, corrected for the effect of the SNP or the GFP by subtracting the mean of the proportions of animals grown at 20 °C when both P0 strains were grown at 25 °C. The raw data are available in Additional file 5: Table S2

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