Fig. 1

Cnidocyte reporter line. a The depiction of the reporter construct used for the generation of the transgenic reporter line. b, c Expression of memOrange2 in planulae of F0 (injected) and F1 (first filial generation) (f–g). Magenta arrowheads point towards examples for positive animals, whereas yellow arrowheads point towards examples for negative animals. Scale bar is 250 μm. d, h Expression of memOrange2 in F0 and F1 primary polyps. Scale bar is 250 μm. e, i Expression of memOrange2 in F0 and F1 juvenile polyps (2.5 months old). Scale bar is 1000 μm. j, k RNA expression and protein localization of memOrange2 and NvNcol-3, revealed by dFISH and immunostaining, in early planulae and tentacles. l A higher magnification capture of immunostaining in a tentacle performed as in k. Scale bar in (j) is 100 μm, 50 μm in k, and 25 μm in l