Fig. 4

Variability between the transcriptional and proteomic expression levels of cnidocyte-expressed genes. This figure shows that the expression patterns of NvNcol-3 and memOrange2 transcripts stained in FastRed (panels a and d) differ from the expression pattern of their protein products stained with Alexa fluor 488 (panels b and e). Examples for cells containing only RNA, only protein or both RNA and protein are indicated by magenta, gray and orange arrowheads, respectively (panels c and f). Quantifications of the cells positive for only RNA, only protein, or both RNA and protein (each from seven animals) are shown as column graphs (panels g and h). i A schematic diagram shows the overlap between the times of gene expression, protein secretion, and protein maturation. Scale bars are 10 μm