Fig. 5

Novel genes with cnidocyte-specific expression. In situ hybridization expression patterns of novel cnidocyte-specific genes identified in this study are shown in late planula and primary polyp. The elongated cells in the ectoderm, seen in the closeup image, can clearly be identified as cnidocytes according to the large unstained space, which is the cnidocyst capsule. Heatmaps of expression levels of each gene in positive (P) and super-positive (SP) cell populations, each across three technical replicates (positives and super-positive indicated as Pos1–3 and negatives as Neg1–3) are also provided. A color code for expression values, ranging from a gradient of red (downregulated) to blue (upregulated), is depicted at the bottom. Scale bars are 100 μm for planulae and primary polyps and 20 μm for closeup panels