Fig. 7

Knockdown of Cnido-Jun results in decreased NvNcol-3 expression. (a) Morpholino antisense oligo design. The MO bases appear in purple, and the sequence of its binding site in the mRNA appears in green. The AUG translation start site is underlined. b Planulae that were injected as zygotes with control MO exhibit normal expression of NvNcol-3. c In contrast, planulae injected as zygotes with Cnido-Jun MO exhibit abnormal expression of NvNcol-3, missing the dense expression domain in the aboral pole and most positive cells lack the typical elongated morphology of NvNcol-3-expressing cells. Panels (d) and (e) present the effects of a second non-overlapping MO against Cnido-Jun and the control MO that was injected in parallel. Oral pole in panels (b–e) are indicated by asterisk, and scale bars are 50 μm. The numbers of embryos exhibiting a normal NvNcol-3 phenotype out of each injected group based on the two indexes described in the text appear below each merged picture