Fig. 1

Prolonged exposure to food perception triggers an inability to recover that is mediated by a transcriptional/translational response. a Diagram of experimental setup with four standard treatment conditions over time. Dimethyl sulfoxide (DMSO; solvent). b Representative images of worm recovery after 3 days post starvation. Arrowheads indicate L1s. c L1 starvation recovery is plotted over time for three biological replicates. d The proportion of larvae that recovered to at least the L4 stage after 3 days of recovery is plotted for three to nine biological replicates. e The proportion of larvae that recovered to at least the L4 stage after 3 days of recovery is plotted for three to nine biological replicates. b–e ***p < 0.001, **p < 0.01; *p<0.05; unpaired t test. Error bars are SEM. Quadruple mutant transgenic background