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Fig. 3 | BMC Biology

Fig. 3

From: Food perception without ingestion leads to metabolic changes and irreversible developmental arrest in C. elegans

Fig. 3

GFP::DAF-16 localization response to food perception is insulin-dependent. a Representative images of how GFP::DAF-16 localization was characterized. b GFP::DAF-16 localization is plotted for three biological replicates. c Average transcript abundance from four biological replicates of mRNA-seq is plotted for selected insulin-like peptides. Nominal p values displayed. d Averages of Pdaf-28::GFP fluorescence intensity normalized by optical extinction per worm using the COPAS BioSorter are plotted for four biological replicates. Exposure to HB101 was 6 h. e Representative images of Pdaf-28::GFP transcriptional reporter gene are presented. f GFP::DAF-16 localization is plotted for three to six biological replicates. g GFP::DAF-16 localization is plotted for three to six biological replicates. The two-way ANOVA interaction p value is listed. h GFP::DAF-16 localization is plotted for three biological replicates. i The proportion of larvae that recovered to at least the L4 stage after 3 days of recovery is plotted for three to four biological replicates. b–i ***p < 0.001, **p < 0.01; *p<0.05; unpaired t test. Error bars are SEM, except for in d where they are standard deviation. a, b, d–i Wild-type background. c Quadruple mutant transgenic background

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