Fig. 4

Food perception significantly affects metabolism but not development. a Overlap between genes significantly affected by food with genes in the metabolic process and larval developmental process GO terms is plotted. b Overlap between genes significantly affected by ivermectin and food with genes in the metabolic process and larval developmental process GO terms is plotted. c The average number of seam cell divisions, out of six possible, is plotted for three biological replicates. Scoring was done 2 days after HB101 addition. d Representative DIC and GFP channel images of fixed worms following Nile red staining are presented. e Quantification of fat droplets in Nile red staining is plotted for three to four biological replicates. c–e ***p < 0.001, **p < 0.01; unpaired t test. Error bars are SEM. a–c Quadruple mutant transgenic background. d, e Wild-type background