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Fig. 3 | BMC Biology

Fig. 3

From: Two-component cyclase opsins of green algae are ATP-dependent and light-inhibited guanylyl cyclases

Fig. 3

Cr2c-Cyclop1 and Vc2c-Cyclop1 are light-inhibited guanylyl cyclase opsins. a Light (532 nm, ~ 20 μW/mm2) and dark activities of Cr2c-Cyclop1 + 1 μM ATR, Cr2c-Cyclop1-YFP + 1 μM ATR, and Cr2c-Cyclop1-YFP without ATR. The cGMP production ability was normalized to one oocyte membrane. n = 3, error bars = SD. b Action spectrum of Cr2c-Cyclop1. n = 3, error bars = SD. c Light sensitivity of Cr2c-Cyclop1 to three different wavelengths of light. Different intensities of blue light (473 nm), green light (532 nm), and orange light (596 nm) were applied. n = 3, error bars = SD. d Light (532 nm, ~ 20 μW/mm2) and dark activities of Vc2c-Cyclop1. One micromolar of ATR was added. The cGMP production ability was normalized to one oocyte membrane. n = 4, error bars = SD. e Action spectrum of Vc2c-Cyclop1. n = 4, error bars = SD. f Light sensitivity of Vc2c-Cyclop1 to 556 nm light. n = 4, error bars = SD. For b, c, e, and f, inhibition percentage was calculated by (dark activity − light activity)/dark activity

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