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Fig. 4 | BMC Biology

Fig. 4

From: Two-component cyclase opsins of green algae are ATP-dependent and light-inhibited guanylyl cyclases

Fig. 4

Characterization of Cr2c-Cyclop1 activity under different conditions. a Dynamic activity of Cr2c-Cyclop1. All three samples were under initial dark stage for 30 s and then put to constant dark (blue square), constant green light illumination (532 nm, ~ 20 μW/mm2, green rhombus), and 3-min green light followed by constant dark (gray dot). Samples were collected and measured at different time points indicated in the figure. n = 3, error bars = SD. b Cr2c-Cyclop1 activity influenced by ATP, Mg2+, and Ca2+. Mg2+ were depleted (no MgCl2), replaced by Ca2+ (5 mM CaCl2) or ATP depleted (no ATP) from the standard reaction buffer. n = 4, error bars = SD. c Cr2c-Cyclop1 activity at different temperatures (10, 20, 30, and 40 °C). n = 3, error bars = SD. d Cr2c-Cyclop1 activity at different pHs (6.3, 7.3, 8.3). n = 3, error bars = SD. Illumination conditions were same for ad

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