Fig. 5

Mutation analysis of Cr2c-Cyclop1. a All mutations were made based on Cr2c-Cyclop1-YFP construct. No ATP, ATP depleted from the standard reaction buffer. With AMP-PNP, 0.25 mM AMP-PNP was added to replace ATP. Illumination condition, 532 nm, ~ 20 μW/mm². Activities of different constructs were adjusted to the same protein amount based on the fluorescence emission value, n = 4, error bars = SD. wt, Cr2c-Cyclop1-YFP wild-type. b Co-expression of Cr2c-Cyclop1-YFP and H352F mutant. Total protein amounts were controlled to be the same based on the fluorescence emission value. Illumination condition, 532 nm, ~ 20 μW/mm². n = 3, error bars = SD. c The relative fluorescence emission values of different constructs were determined to ensure similar total protein amount. n = 3, error bars = SD