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Fig. 2 | BMC Biology

Fig. 2

From: A Label-free Multicolor Optical Surface Tomography (ALMOST) imaging method for nontransparent 3D samples

Fig. 2

Color and shape in test samples. Color code on a resistor. a Photograph of the resistor. b 3D reconstruction of the blue channel. c 3D reconstruction of the green channel. d 3D reconstruction of the red channel. e Relative intensity profile of the color channels as indicated in bd. f 3D RGB color reconstruction. Small artifacts like the flare on the thread above the actual resistor are a consequence of extended reflexes of the reflective metal part connecting the resistor. The brown, green, red, and gold color rings (from top to bottom) imprinted on the resistor, part of the four color code used to describe its properties, can be revealed. The color balance for the three colors was adapted manually. Seed cone sample (Metasequoia glyptostroboides) with complex surface structure. g Photograph of the seed cone. h Individual image from the ALMOST device (blue channel). i Reconstructed sagittal section through a central plane of the seed cone (blue channel). j Intensity profile along the line indicated in i to compare inside and outside of the complex shape. k, l 3D semitransparent volume rendering of the seed cone in three colors (colors inverted compared to j, for realistic color display). The color balance for the three colors was adapted manually. Images show different angles, including a view from below the cone, showing that complex samples can be imaged with ALMOST. Scale bars = 2 mm. Imaging conditions are summarized in Additional file 20: Table S1

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