Skip to main content

Advertisement

Fig. 4 | BMC Biology

Fig. 4

From: A Label-free Multicolor Optical Surface Tomography (ALMOST) imaging method for nontransparent 3D samples

Fig. 4

Combining ALMOST and fluorescence OPT on adult Drosophila. A mutant fruit fly expressing GFP in the eyes is imaged. a shows the reflective image acquired by ALMOST in the blue channel. b Section through reconstruction of the fly in reflective mode (front view, blue channel). c 3D rendering of the fly imaged in reflective mode. d Combination of the 3D rendering of the reflective and the fluorescence modes. e Single three-color (raw) ALMOST image before reconstruction and rendering of a red-eyed wild-type Drosophila head. f ALMOST 3D reconstruction of a sequence of rotational images as in e. g Single three-color ALMOST image (before reconstruction and rendering) of a glace-eyed mutant Drosophila head with narrowed eyes of reduced size. h ALMOST 3D reconstruction of a sequence of rotational images as in g. Scale bars = 500 μm. Imaging conditions are summarized in Additional file 20: Table S1

Back to article page