A Label-free Multicolor Optical Surface Tomography (ALMOST) imaging method for nontransparent 3D samples

Table 1 Comparing ALMOST with other common 3D imaging techniques. + and − signs are indicating how well the different techniques are suited for the challenges in the category listed in the columns on the left side

	ALMOST	CT	Light Sheet	OPT	Confocal
Labeling
Label-free imaging	++	++	−(+)	+	+
Fluorescence	+	−	++	+	++
Multiplexing	+	−	+	+	+
Data
Isotropic	++	++	+	++	−
3D	++	++	++	++	+
Color	+	−	−	+	+
Samples
Live	++	+	++	++	+
Mesoscale	+	++	+	+	−
Microscale	−	+	+	−	++
Opaque	++	++	−	−	−(+)
(Semi-) transparent	++	++	+	+	+
Scattering	+	++	−	−	−

ISSN: 1741-7007