Fig. 5

Loss of atfs-1 reduces increased resistance to multiple stresses in long-lived nuo-6 worms and decreases stress resistance in wild-type worms. a Sensitivity to oxidative stress was assessed by exposing worms to plates containing 2 mM paraquat (PQ). nuo-6 worms showed increased resistance to oxidative stress, which was completely abolished by removal of atfs-1. b Sensitivity to osmotic stress was assessed by exposing worms to 550 mM NaCl and quantifying survival after 48 h. nuo-6 worms exhibit increased survival compared to wild-type worms. Loss of atfs-1 resulted in decreased survival in both wild-type and nuo-6 worms. c To measure sensitivity to heat stress, worms were incubated at 37 °C and survival was monitored hourly. nuo-6 worms exhibit increased survival compared to wild-type worms. In both cases, heat stress survival was decreased by loss of atfs-1. d Deletion of atfs-1 causes increased sensitivity to anoxia in both wild-type and nuo-6 worms. Error bars indicate SEM. In panels A and C, p value indicates significance between nuo-6 and nuo-6;atfs-1 worms. **p < 0.01, ***p < 0.001. See Additional file 5: Table S5 for details on N and replicates